Spectrofluorimetric determination of individual bile acids in biological fluids: duodenal content and bile.

A micromethod is described for the separation and quantitative determination of individual bile acids in duodenal content and bile. After a mild saponification procedure for splitting the taurine and glycine conjugates, the products are separated by thin-layer chromatography on silicagel layer with the solvent system //0-octane-ethyl acetate-acetic acid (60 ml + 35 ml + 5 ml) and determined by spectrofluorirrietry. A series of control experiments has been performed on the validation of the procedure. Values obtained for human subjects or experimental applications of the method are presented.